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Image Search Results
Journal: Nature chemistry
Article Title: An automated platform for the enzyme-mediated assembly of complex oligosaccharides
doi: 10.1038/s41557-019-0219-8
Figure Lengend Snippet: a , Sulfonated tags 1a - c bearing an methyl hydroxylamine moiety can be condensed with reducing glycans to give compounds such as 2a-c . After a glycosyltransferase-catalyzed reaction to give compounds such as 3a-c , the sulfonate moieties of the tag allow product purification by DEAE solid phase extraction (SPE). b , Various steps for automated glycosyltransferase-mediated oligosaccharide assembly. The addition of reagents, pH adjustment, withdrawal of samples, transfer of reaction mixtures, and SPE are performed by a 4-needle head mounted on a robotic arm. c , Schematic illustration of the automation platform. A. 4-needle head with pH needle mounted on a robotic arm for liquid handling. B. Four syringe pumps of different capacity to drive liquid handling. C. Temperature controlled shaking rack to hold reaction vessels (50 × 15 mL vials). D. Temperature controlled rack (4 °C) for reagent storage (96 × 8 mL vials). E and F. SPE racks for compound purification (80 × 1 mL cartridges and 80 × 3 mL cartridges). G. Reagent rack (30 × 60 mL vials). H. Sample rack for analysis intermediate compounds (160 × 2 mL vials). I. ISYNTH AI SWING platform.
Article Snippet: A glycosynthesizer was built based on
Techniques: Purification, Extraction